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  1. Advances in Microbial Food Safety, Volume 2 by Sofos | | Booktopia
  2. The impact of next-generation sequencing on food safety
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Advances in Microbial Food Safety, Volume 2 by Sofos | | Booktopia

This Reference is not available in your current subscription. Notify your administrator of your interest. Advances in Microbial Food Safety, Volume 1 Details New research, outbreaks of foodborne disease and changes to legislation mean that food microbiology research is constantly evolving. This book summarises the key trends in this area for the food industry. The book begins with an introductory chapter discussing food safety management systems from the past to the present day and looking to future directions. The book moves on to provide updates on specific pathogens including Salmonella, Listeria monocytogenes and Bacillus species.

New developments in the area are explored with chapters on emerging parasites in food, advances in separation and concentration of microorganisms from food samples, new approaches in microbial pathogen detection, and an update on novel methods for pathogen control in livestock preharvest. Show less.

View More. Back to Table of Contents. New in Food Science. Carbohydrate Chemistry for Food Scientists 3rd Edition BeMiller, James N. Open Share Save. Click here to Expand all. Click here to Collapse all.

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View Section, Front Matter. View Section, Table of Contents. View Section, Part I. Expert Interview with Dr R. Bruce Tompkin. View Section, 1. View Section, Part II.

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  • Pathogen Updates. View Section, 2.

    The impact of next-generation sequencing on food safety

    Pathogen Update: Salmonella. View Section, 3. WGS has been shown to discriminate between highly clonal pathogens and specific subtypes, e. Thus, WGS is more accurate for comparing isolates and has the added advantage of being faster than traditional, culture-dependent techniques. Besides identifying causative pathogens, WGS can be used to predict traits such as virulence and antimicrobial resistance. These sequences were deposited publicly, enabling the rapid development of PCR assays that could specifically detect the outbreak strain.

    Although initially used retrospectively to analyze outbreak-causing pathogens, WGS of microbial pathogens in now increasingly applied to the prospective surveillance of foodborne microbes in the USA, UK, and other parts of Europe.

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    Besides identifying all of the microbes in a sample, metagenomics and transcriptomics can be used to determine isolate traits without the need for culture. Such data has significant potential for the rational development of new microbial control compounds and strategies. There are, however, several challenges regarding the use of NGS for food safety. NGS technologies also remain relatively expensive, which prevents access by less-developed countries and may hinder implementation for routine use.

    Besides NGS, many other technologies such as electrochemical-, optical-, and nanomaterial-based biosensors have been developed to detect microbial pathogens, although these are typically focused on the detection of single, specific pathogens. Nanotechnology is another field of research that will likely have a major impact on food safety. Silver nanoparticles are already commercially used for their high antimicrobial activity, while gold nanoparticles have been widely studied for incorporation in biosensors.

    However, safety concerns remain regarding the accumulation of nanomaterials in the human body and the environment. Food packaging is used to facilitate product handling, preserve nutritional value, reduce spoilage, and extend product shelf life.

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    Smart packaging technologies could potentially provide additional functions such as communicating measurements of freshness, temperature, and microbial growth in real time. Microbial contamination can be detected by, among others, changes in gas composition within sealed packaging, changes in pH, and the release of volatile compounds.

    This sensor uses antitoxins immobilized on RFID tags that are incorporated within food packaging. Each sensor can be connected to a wireless network to provide real-time monitoring. The focus on minimal processing has led to growing implementation of non-thermal preservation technologies such as high hydrostatic pressure HHP , ultraviolet UV radiation, and cold plasma CP treatments. However, as with all food preservation technologies, the possibility of microbial survivors remains.

    Furthermore, viruses are more likely to survive such treatments, and at present, we do not have reliable tools to confirm virus inactivation. Another emerging trend in food preservation is the use of natural antimicrobial compounds, including ethanol, spices, and essential oil extracts of plants such as oregano, rosemary, and garlic. For food safety to fully benefit from NGS technologies, we require well-curated NGS databases of food-associated microbes. As Dr. We believe that regulators and food manufacturers have an important role to play in working together to fully embrace new technologies and drive transparency through data sharing.

    Such an approach could help maximize the value that new technology can bring in protecting the safety of the entire food chain. Microbial food contamination poses a serious risk to human health that is associated with significant economic losses. The increasing globalization of the food trade and trends in consumer preferences will continue to place demands on the development of new and improved methods for ensuring food safety, as well as the drafting of matching regulation.

    Bedale, while Dr. Robust food safety management practices that ensure food is safe at all stages of production are more important than ever before. World Health Organization. Food safety in the 21st century. Biomedical Journal. Foodborne viruses: detection, risk assessment, and control options in food processing. International Journal of Food Microbiology. Epidemiological evidence and health risks associated with agricultural reuse of partially treated and untreated wastewater: A review.

    Frontiers in Public Health. Food safety challenges and One Health within Europe. Acta Veterinaria Scandinavica. The use of next generation sequencing for improving safety: Translation into practice. Food Microbiology. Omics approaches in food safety: fulfilling the promise? Trends in Microbiology.